Agilent 1260 Infinity HPLC  

Manufacturer: Agilent
Model: 1260
Agilent 1260 HPLC
Includes the Following Components
G1315D 1260 DAD VL
G1316A 1260 TCC
G1364C 1260 FC-AS
G1311B 1260 Quat Pump
G1329B 1260 ALS,

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Used Agilent 1260 LC System with Quaternary Pump and VWD detector  

This  Agilent 1260 LC System with Quaternary Pump and VWD detector included:
1260 Solvent Tray
1260 Quaternary Pump G1311C
1260 Autosampler G1329B
1260 Thermostatted Column Compartment
1260 VWD G1314F with standard Flow Cell
 

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Used Agilent 1260 HPLC with Thermostat for Autosampler , Binary Pump SL ,DAD  

This Agilent 1260 HPLC included:
1260 Binary Pump SL and G4225A
1260 HiP Degasser with solvent tray  or G1311B(built-in degasser )
Thermostatted Column Compartment (TCC SL+)
 Diode Array Detector (DAD),
 Agilent 1260
Autosampler (HiP-ALS SL)
 1260 Thermostat for Autosampler
If you are interested in Agilent 1260 HPLC with Thermostat for Autosampler , Binary Pump SL ,DAD,please contact us.

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Used Agilent 1260 HPLC with 1260 with Binary pump,DAD,Autosampler, Degasser with solvent tray  

This Agilent 1260 HPLC included:
Binary pump with switch valve
 1260 Binary Pump
 Thermostatted Column Compartment (TCC SL+)
 Diode Array Detector (DAD)
 Autosampler (HiP-ALS SL)
 1290 Thermostat for Autosampler
1260 HiP Degasser with solvent tray
 

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Used Agilent 1260 Infinity HPLC System with DAD,Quat Pump,ALS,TCC  

This Agilent 1290 Infinity Series HPLC System included:
1260: DAD
1260: Quat Pump (with built in Degasser); 600 Bar
1260: ALS
1260: TCC
W7 Pro Computer
Chemstation Software
 

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Agilent HPLC 1260  

The Agilent 1260 Infinity HPLC-Chip/MS system is a re-useable microfluidic chip-based technology for high sensitivity nanospray LC/MS. The Agilent 1260 replaced the Agilent 1200 and delivers results faster to save time and money. 

Agilent Technologies 1260 Series

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agilent 1260 hplc

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This Agilent 1260 HPLC included: Binary pump with switch valve 1260 Binary Pump Thermostatted Column Compartment (TCC SL+)

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Hplc Agilent Technologies 1260 Infinity II LC System Brochures  

Prove Solutions for Clnical Research and Forensic Toxicology
See Agilent's clinical research products and services for specific research applications.

16-Sep-2016
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Agilent InfinityLab. Maximize your LC Workflow Efficiency.
Agilent InfinityLab. Maximize your LC Workflow Efficiency.

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Agilent InfinityLab. Maximize your LC workflow Efficiency.
Agilent InfinityLab. Maximize your LC workflow Efficiency.

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Agilent InfinityLab. Maximum efficiency for your liquid chromatography workflows.
Agilent InfinityLab. Maximum efficiency for your liquid chromatography workflows.

20-Jun-2016
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Agilent InfinityLab Supplies for the Agilent 1260 Infinity II LC with Multisampler
Agilent InfinityLab S

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HPLC Column | Agilent ZORBAX Extend-C18 Small Molecule Separations LC Column  

Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH - up to pH 11.5. Columns are best applied for separations of compounds that are either (1) basic and have little or no retention at low or intermediate pHs, (2) more stable or more soluble at high pH, or (3) basic and show poor peak shape at low or intermediate pH.
Features Of Agilent ZORBAX Extend-C18 Small Molecule Separations LC Column:
High efficiency and long life at high pH - up to pH 11.5
Unique bidentate bonding and double endcaping provides high pH stability
More efficiency and better peak shape than polymer-based columns
Improve retention, resolution and peak shape of basic compounds
High sensitivity for LC/MS sepa

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Agilent 1260 Infinity II LC System  

Infinitely more confident.
The new 1260 Infinity LC sets higher standards in performance and value to give you more confidence in your results. The 600 bar power range combines with 80 Hz UV detector speeds and up to 10 times higher sensitivity. That‘s true UHPLC performance! At the same time you can run your existing HPLC methods completely unchanged. In short, the best solution for any HPLC or UHPLC application.
And within the price range of a typical HPLC!


Raising the HPLC standard 

The 1260 Infinity LC raises the bar in HPLC! With a 600 bar power range up to 5 mL/min for isocratic, quaternary and binary systems  and 200 bar up to 10 mL/min for isocratic and quaternary systems – plus 80 Hz detector speeds and 10 x higher sensitivity, you will reach new levels of versati

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Hplc Agilent Technologies 1260 Infinity II LC System  

Infinitely more confident.
The new 1260 Infinity LC sets higher standards in performance and value to give you more confidence in your results. The 600 bar power range combines with 80 Hz UV detector speeds and up to 10 times higher sensitivity. That‘s true UHPLC performance! At the same time you can run your existing HPLC methods completely unchanged. In short, the best solution for any HPLC or UHPLC application.
And within the price range of a typical HPLC!


Raising the HPLC standard 

The 1260 Infinity LC raises the bar in HPLC! With a 600 bar power range up to 5 mL/min for isocratic, quaternary and binary systems  and 200 bar up to 10 mL/min for isocratic and quaternary systems – plus 80 Hz detector speeds and 10 x higher sensitivity, you will reach new levels of ve

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1100 HPLC  

Introduced in 1995, Agilent's 1100 series HPLC system is considered the world's most popular HPLC. They are incredibly robust machines that can last a long time. Finding a used Agilent 1100 on LabX is easy and maintenance for older machines consists of changing seals, filter solvents, lamps, and the odd part in the vacuum degasser. Parts from the Agilent 1050 are often shared with the Agilent 1100 which make it easy to maintain.

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Agilent Technologies 1100 Series

hplcagilent1100.hplchplc.com/

Used Agilent 1100 Series HPLC with Multiple Wavelength Detector System. 1100 hplc,agilent hplc,hplc system,used hplc,used agilent hplc. HPLC System with ...

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Used Agilent 1100 S

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Agilent HPLC  

Find great deals on HPLCHPLC.COM for Used and Refurbished  Agilent HPLC in Analytical Lab Instruments. Shop with confidence. 





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agilent 1200 hplc


hplc.hplchplc.com/list/agilent+1200+hplc.html
 
This HP/Agilent 1200 Series Nano HPLC system has just been moved from a working lab. It comes with the followings: G1379B Degasser with Tray G2226A ...
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Agilent Technologies 1260 Series


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USED AGILENT HP 1200 HPLC Series SYSTEM


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used

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features Of Agilent 1260 Infinity II Variable Wavelength Detector:
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resoluti

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Agilent 1260 Infinity II Multiple Wavelength LC Detector  

Reliable, simultaneous quantification of primary compounds, by products and impurities
The diode array design of the Agilent 1260 Infinity II Multiple Wavelength Detector offers very low detector noise (less than ± 7 μAU) for precise quantification of trace levels, regardless of the number of signals recorded. Over a wavelength range from 190 to 950 nm, simultaneous detection of up to eight compound-specific wavelengths for optimum selectivity is provided. High-speed UV detection with up to 120 Hz data rates keeps pace with the analysis speed of fast LC.
Features:
Increased sensitivity and selectivity - with simultaneous acquisition of up to eight compound-specific wavelengths.
Low detection limits - low noise front-end electronics and special flow cell design deliver lowest detection

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1290 HPLC  

The Agilent 1290 Infinity LC system provides the highest levels of speed, resolution, flexibility and sensitivity for any LC and LC/MS application. 

 

Used Agilent 1290 Infinity Series HPLC System

hplcagilent1290.hplchplc.com/page-461081.html

 

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1290 Infinity Series HPLC

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 Used Agilent 1290 Infinity Series HPLC System · Download Hplc Agilent Technologies1290 Infinity II LC System Brochure and Guide. Downloa

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What Is Ultra Performance Liquid Chromatography (UPLC Technology)?  

In 2004, further advances in instrumentation and column technology were made to achieve very significant
increases in resolution, speed, and sensitivity in liquid chromatography. Columns with smaller particles [1.7
micron] and instrumentation with specialized capabilities designed to deliver mobile phase at 15,000 psi [1,000
bar] were needed to achieve a new level of performance. A new system had to be holistically created to perform
ultra-performance liquid chromatography, now known as UPLC technology.
Basic research is being conducted today by scientists working with columns containing even smaller 1-microndiameter
particles and instrumentation capable of performing at 100,000 psi [6,800 bar]. This provides a glimpse
of what we may expect in the future.

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HPLC Column | Agilent LiChrospher Small Molecule Separations LC Column  

Agilent LiChrospher silica columns are made with spherical, 'sil' type porous silica particles. LiChrospher 60 RP-select B starts with 60Å pore size silica optimized for symmetrical peak shapes of basic compounds. LiChrospher 100Å is offered in both C8 and C18 as well as an endcapped C18. These columns are noted for high sample capacity and efficiency. The popular LiChrospher packings are offered in the convenient and economical Agilent cartridge configuration. For normal phase chromatography, Agilent supplies polar modified silica gel phases LiChrospher Si, LiChrospher NH2 , LiChrospher DIOL and LiChrospher CN as convenient cartridge columns.
Features Of Agilent LiChrospher Small Molecule Separations LC Column:
Available in 5 µm particle size
Reversed-phase available in RP-C18, RP-18

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features :
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resolution gainin fast LC -at up to 120 Hz data acquisition r

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HPLC Column | Agilent ZORBAX Eclipse XDB-CN Small Molecule Separations LC Column  

For normal-phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings.
Features:
Made from highly pure Rx-SIL
Excellent choice for normal-phase applications with basic compounds
Equilibrates more rapidly than ZORBAX Rx-Sil and is used for many of the same normal-phase applications
To compare technical information and specifications for different phases, see our phase specifications chart.

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AGILENT HP 1200 HPLC Series SYSTEM  

1200 Series HPLC System in excellent working condition.
Includes:
    •    Sold Guaranteed in Good Working Condition
    •    Tested and Verified in Good Working Condition prior to ship
•    G1322A Agilent 1200 Micro Vacuum Degasser
•    Your choice Binary or Quaternary Pump
•    Many AutoSamplers to choose from
•    G1316B Agilent 1200 Thermostatted Column Compartment
•    G1315D Agilent 1200/1260 DAD Diode Array Detector
•    Agilent Solvent tray and bottles
•    All fittings, cables, and tubing
•    Agilent Chemstation Software with License
•    Computer
•    21.5" LCD Monitor
•    Keyboard and mouse
    •    WARRANTY: 3 Months Parts and Labor
INSTALLATION on-site in USA.
Includes:
• Pump sea

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USED AGILENT HP 1200 HPLC Series SYSTEM  

We offer an Agilent Technologies 1200 Series HPLC System in excellent working condition. Includes:
    •    Tested and Verified in Good Working Condition prior to ship
    •    G1322A Agilent 1200 Micro Vacuum Degasser
    •    Your choice Binary or Quaternary Pump
    •    Many AutoSamplers to choose from
    •    G1316B Agilent 1200 Thermostatted Column Compartment
    •    G1315D Agilent 1200/1260 DAD Diode Array Detector
    •    Agilent Solvent tray and bottles
    •    All fittings, cables, an

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Column Chromatography is the the first form of techniques developed in chromatography!  

Column chromatography is the prototype or the basic type of chromatography. It was the first form of techniques developed in chromatography. One can easily explain the principle and procedure of chromatography using it.
Other types of chromatography techniques like high performance liquid chromatography (HPLC), gas chromatography (GC), paper chromatography were developed with column chromatography as a module and making slight variations.
In-spite of many advanced methods of chromatography, still this method of chromatography is widely used in research and industry.
This chromatography is basically a type of adsorption chromatography techniques.

Here the separation of components depends upon the extent of adsorption to stationary phase. Here the stationary phase is a polar solid materia

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History Of Chromatography  

Chromatography was first employed in Russia by the Italian-born scientist Mikhail Tsvet in 1900. He continued to work with chromatography in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll,carotenes, and xanthophylls. Since these components have different colors (green, orange, and yellow, respectively) they gave the technique its name. New types of chromatography developed during the 1930s and 1940s made the technique useful for many separation processes.
Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won a Nobel prize.They established the principles and basic techniques of partition chromatograph

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HPLC Column | Agilent HC-C18(2) Small Molecule Separations LC Column  

Agilent HC-C18(2) columns are an excellent choice for separations of many types of compounds.
Agilent HC-C18(2) is a more retentive C18 with a higher carbon load. An excellent value alternative to other high carbon load columns, it also provides superior peak shape for basic compounds.
Features Of Agilent HC-C18(2) Small Molecule Separations LC Column:
Higher carbon load - 17% - provides greater retention for moderately polar and non-polar compounds
Ideal for non-polar compounds and separations that start at mid-level % organic (at least greater than 10% organic)
Good choice for industrial samples or samples dissolved in organic/mostly organic solvents
Stable over a very wide pH range (2-9) for maximum flexibility
To compare technical information and specifications for different pha

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Displacement Chromatography  

The basic principle of displacement chromatography is: A molecule with a high affinity for the chromatography matrix (the displacer) will compete effectively for binding sites, and thus displace all molecules with lesser affinities. There are distinct differences between displacement and elution chromatography. In elution mode, substances typically emerge from a column in narrow, Gaussian peaks. Wide separation of peaks, preferably to baseline, is desired in order to achieve maximum purification. The speed at which any component of a mixture travels down the column in elution mode depends on many factors. But for two substances to travel at different speeds, and thereby be resolved, there must be substantial differences in some interaction between the biomolecules and the chromatography m

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How to Prevent Mobile Phase Problems in HPLC  

Low sensitivity and rising baselines, noise, or spikes on the chromatogram can often be attributed to the mobile phase. Contaminants in the mobile phase are especially troublesome in gradient elution. The baseline may rise, and spurious peaks can appear as the level of the contaminated component increases. Water is the most common source of contamination in reversed phase analyses. You should use only high purity distilled or deionized water when formulating mobile phases. However, several common deionizers introduce organic contaminants into the water. To remove these contaminants, pass the deionized water through activated charcoal or a preparative C18 column. Use only HPLC grade solvents, salts, ion pair reagents, and base and acid modifiers. Cleaning lower quality solvents is time cons

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Partition Chromatography  

Partition chromatography was one of the first kinds of chromatography that chemists developed. The partition coefficient principle has been applied in paper chromatography,thin layer chromatography, gas phase and liquid–liquid separation applications. The 1952Nobel Prize in chemistry was earned by Archer John Porter Martin and Richard Laurence Millington Synge for their development of the technique, which was used for their separation of amino acids. Partition chromatography uses a retained solvent, on the surface or within the grains or fibers of an "inert" solid supporting matrix as with paper chromatography; or takes advantage of some coulombic and/or hydrogen donor interaction with the stationary phase. Analyte molecules partition between a liquid stationary phase and the eluent.

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principle of hplc:High Performance Liquid Chromatography (HPLC)  

High performance liquid chromatography (HPLC) has become a very versatile and powerful separation and analytical method over the years. It is an advanced form of liquid chromatography (LC).
Instead of introducing the solvent into the column and allowing it to drip down under the influence of gravity, in HPLC the sample is forced through the column under high pressures of nearly 400 atm, resulting in faster and more efficient separation.
This technique is also called high pressure liquid chromatography.

The Principle of HPLC
HPLC follows the same basic principle as chromatography. Different components in the sample have varying affinities to the adsorbent material. This causes a difference in the flow rate for each component which leads to their separation as they come out of the column.

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Evaporative Light Scattering Detector (ELSD)  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds where UV detection might be a restriction and therefore compounds does not very efficient absorb UV radiation, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols. ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI).

ELSD | Evaporative Light Scattering Detector

elsd.hplchplc.com/

 

... Scattering Detector. An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC).

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P

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chromatography  

Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.

Chromatography

chromatography.hplchplc.com/ 
 







Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.


Partition Chromatography


chromatography.hplchplc.com/page-461157.html
 
Partition chromatography was one of the first k

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waters hplc  

Whatever your application, Alliance® HPLC, BreezeTM 2 HPLC, and AutoPurificationTM systems offer you proven solutions for all of your HPLC requirements - for today and the future. These systems are synonymous with dependable, routine performance and versatility.

Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance HPLC family meets the rigorous requirements of routine chromatography and the performance standards of new-product research and method development.

Breeze 2 HPLC Comes with a pump, detector, injector, and is pre-configured for different levels of HPLC. It includes Breeze Software, an intuitive interface that is easy to set-up, learn, and use.
waters


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Waters Alliance HPLC THAT WORKS AS HARD AS YOU

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Troubleshooting HPLC- Tailing Peaks  

As in the previous posts for this series, these suggestions assume that you are working with an established method and had successful results in the past. If a method is rugged, it is developed to minimize tailing by using a stationary column phase that is base-deactivated as well as appropriate modifiers and pH adjustment in the mobile phase as needed.  Keep in mind that if the method is not as rugged as it should be, change(s) in the method might be advised if tailing continues to be an ongoing problem.

With troubleshooting, as always, it is critical to note when the change occurred and how it might correlate to changes in the system.  Equally important is to change one thing at a time to identify the source(s) of difficulty. The following are examples of things that could contribute

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Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes  

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties.
When some, but not all of your peaks are low, and you are analyzing multiple analytes, the number one rule is to look for trends. As mentioned in the previous post for this series, when you have only one analyte, or a handful of very similar analytes, the following may also apply. Again in this post, the assumption is that you are using an established working method and that you see a loss in response for every sample or standard that is injected. If you are only seeing the problem with samples and not your quantitation

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Principle of HPLC:High Performance Liquid Chromatography (HPLC) : Principle, Types, Instrumentation and Applications  

Chromatography is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition. This involves a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it. Sample components that display stronger interactions with the stationary phase will move more slowly through the column than components with weaker interactions. This difference in rates cause the separation of variuos components. Chromatographic separations can be carried out using a variety of stationary phases, including immobilized silica on glass plates (thin-layer chromatography), volatile gases (gas chromatography

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principle of hplc:How Does High Performance Liquid Chromatography Work?/what is Detector,Chromatogram?  

How Does High Performance Liquid Chromatography Work?

The components of a basic high-performance liquid chromatography [HPLC] system are shown in the simple diagram in Figure E.
A reservoir holds the solvent [called the mobile phase, because it moves]. A high-pressure pump [solvent delivery system or solvent manager] is used to generate and meter a specified flow rate of mobile phase, typically milliliters per minute. An injector [sample manager or autosampler] is able to introduce [inject] the sample into the continuously flowing mobile phase stream that carries the sample into the HPLC column. The column contains the chromatographic packing material needed to effect the separation. This packing material is called the stationary phase because it is held in place by the column hardware.

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Shimadzu Evaporative Light Scattering LC Detector LTII (ELSD-LTII)  

In the history of high-performance liquid chromatographs, which dates to the early 1960s, refractive index detectors (RI detectors) have often been used as general-purpose detectors. RI detectors enable the detection of components that do not possess UV absorbance and give a proportional relationship between the heights of detected peaks and the quantities of detected components. So, in comparison with absorbance detectors (UV detectors), they offer advantages such as the ability to ascertain unknown component quantities and obtain molecular weight distributions for macromolecules. On the other hand, they also have various disadvantages. For example, they cannot be used for gradient analysis, the baselines they produce are susceptible to the influence of fluctuations in the ambie

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