HPLC instrumentation and chromatography principle  

HPLC instrumentation and chromatography principle

This HPLC chromatography lecture explains the HPLC principle and instrumentation.
 
https://www.youtube.com/embed/pGYXd4k5F9o
 

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What Is Ultra Performance Liquid Chromatography (UPLC Technology)?  

In 2004, further advances in instrumentation and column technology were made to achieve very significant
increases in resolution, speed, and sensitivity in liquid chromatography. Columns with smaller particles [1.7
micron] and instrumentation with specialized capabilities designed to deliver mobile phase at 15,000 psi [1,000
bar] were needed to achieve a new level of performance. A new system had to be holistically created to perform
ultra-performance liquid chromatography, now known as UPLC technology.
Basic research is being conducted today by scientists working with columns containing even smaller 1-microndiameter
particles and instrumentation capable of performing at 100,000 psi [6,800 bar]. This provides a glimpse
of what we may expect in the future.

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HPLC (high performance liquid chromatograph ) Instrument Operation  

General components of a high performance liquid chromatograph.
HPLC solvent delivery systems.
How automatic injectors work.
Common HPLC detectors.
Downlod powerpoint file about hplc instrumentation .
source:Philadelphia University

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Principle of HPLC:High Performance Liquid Chromatography (HPLC) : Principle, Types, Instrumentation and Applications  

Chromatography is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition. This involves a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it. Sample components that display stronger interactions with the stationary phase will move more slowly through the column than components with weaker interactions. This difference in rates cause the separation of variuos components. Chromatographic separations can be carried out using a variety of stationary phases, including immobilized silica on glass plates (thin-layer chromatography), volatile gases (gas chromatography

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HPLC (high performance liquid chromatograph ) Instrumentation  

Main components in an HPLC system include the solvent reservoir, or multiple reservoirs, a high-pressure pump, a column, injector system and the detector.

The reservoir holds the solvent, which is referred to as the mobile phase because it moves. There are usually a minimum of two reservoirs in a system, with each holding up to 1000 cc of solvent and usually fitted with a gas diffuser through which helium can be bubbled. A pump is used to generate a specified flow of the mobile phase. Although manual injection of samples is still possible, most HPLCs are now fully automated and controlled by computer. The injector, or auto sampler, introduces the solvent into a phase stream that carries the sample into the high pressure (up to 400 bar) column, which contains specific packing material nee

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Features Of Agilent 1290 Infinity II LC System  

Maximize Analytical Efficiency
More chromatographic resolution – specially designed components in the sample flow path achieve lowest system dispersion.
Higher peak capacity for challenging separations ­– switch easily between single dimension UHPLC and the ultimate chromatographic power of 2D-LC.
Lower carryover for uncompromised data quality – multiwash capabilities of the 1290 Infinity II Multisampler reduce carryover to less than 10 ppm even for challenging compounds such as chlorohexidine.
Unique detection capabilities - combine lowest detection limits with an ultra-wide dynamic range by using the new 1290 Infinity II HDR- DAD or 1290 Infinity II ELSD.
Maximize Instrument Efficiency
Faster injection cycles with dual-needle injection and smart overlapped injections off

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Hplc Agilent Technologies 1260 Infinity II LC System Brochures  

Prove Solutions for Clnical Research and Forensic Toxicology
See Agilent's clinical research products and services for specific research applications.

16-Sep-2016
1.71 MB
PDF


Agilent InfinityLab. Maximize your LC Workflow Efficiency.
Agilent InfinityLab. Maximize your LC Workflow Efficiency.

15-Aug-2016
572.54 KB
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Agilent InfinityLab. Maximize your LC workflow Efficiency.
Agilent InfinityLab. Maximize your LC workflow Efficiency.

26-Jul-2016
884.18 KB
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Agilent InfinityLab. Maximum efficiency for your liquid chromatography workflows.
Agilent InfinityLab. Maximum efficiency for your liquid chromatography workflows.

20-Jun-2016
4.26 MB
PDF


Agilent InfinityLab Supplies for the Agilent 1260 Infinity II LC with Multisampler
Agilent InfinityLab S

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Waters ACQUITY Arc HPLC and UHPLC System  

HPLC and UHPLC method compatibility at the flip of a switch

The ACQUITY Arc System is a quaternary-based, modern LC system for scientists working with established methods who are looking for the versatility and robustness required to bridge the gap between HPLC and UPLC while continuing to support validated assays.

True plug-and-play method compatibility and the ability to switch between HPLC and UHPLC separations at the flip of a switch with Arc Multi-flow path Technology
Get mass detection, without changing the way you work, with the ACQUITY QDa Detector
Deploy the benefits of 2.x μm particle columns to boost the chromatographic performance of your methods
On-demand mobile phase creation with Auto●Blend Plus Technology, eliminating the cumbersome and error-prone appr

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chromatography  

Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.

Chromatography

chromatography.hplchplc.com/ 
 







Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.


Partition Chromatography


chromatography.hplchplc.com/page-461157.html
 
Partition chromatography was one of the first k

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Agilent 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

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Hplc Agilent Technologies 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

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History Of Agilent  

Agilent Technologies was created in 1999 by the spin-off of Hewlett-Packard's (HP's) "Medical Products and Instrument Group",[including instrumentation and chemical analysis, electronic component and medical equipment product lines. The split was predicated on the difficulty of growing HP's revenue stream and on the competitive vigor of smaller, more agile competitors.The company's launch slogan was "Innovating the HP Way", which capitalized on the strong HP corporate culture.The starburstlogo was selected to reflect "a burst of insight" (or "spark of insight")and the name "Agilent" aimed to invoke the notion of agility as a trait of the new firm.The Agilent spin-off was accompanied by an initial public offering which raised US$2,100,000,000, setting a record at the time.
2000–2008
In

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