Used Agilent 1260 HPLC with Thermostat for Autosampler , Binary Pump SL ,DAD  

This Agilent 1260 HPLC included:
1260 Binary Pump SL and G4225A
1260 HiP Degasser with solvent tray  or G1311B(built-in degasser )
Thermostatted Column Compartment (TCC SL+)
 Diode Array Detector (DAD),
 Agilent 1260
Autosampler (HiP-ALS SL)
 1260 Thermostat for Autosampler
If you are interested in Agilent 1260 HPLC with Thermostat for Autosampler , Binary Pump SL ,DAD,please contact us.

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Used Agilent 1260 HPLC with 1260 with Binary pump,DAD,Autosampler, Degasser with solvent tray  

This Agilent 1260 HPLC included:
Binary pump with switch valve
 1260 Binary Pump
 Thermostatted Column Compartment (TCC SL+)
 Diode Array Detector (DAD)
 Autosampler (HiP-ALS SL)
 1290 Thermostat for Autosampler
1260 HiP Degasser with solvent tray
 

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Used Agilent 1260 LC System with Quaternary Pump and VWD detector  

This  Agilent 1260 LC System with Quaternary Pump and VWD detector included:
1260 Solvent Tray
1260 Quaternary Pump G1311C
1260 Autosampler G1329B
1260 Thermostatted Column Compartment
1260 VWD G1314F with standard Flow Cell
 

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Used Agilent 1100 with Binary Pump  

Manufacturer: Agilent Technologies
Condition: As Is
Includes:

1100 Solvent Tray
1100 Binary Pump
1100 Vacuum Degasser
1100 Autosampler
Cables and Manuals
Used Agilent 1100 LC System with Binary Pump & DAD is currently not available for ordering, and it may not be in stock again.

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 Used Agilent 1200 Hplc System with Binary Pump  

This HP/Agilent 1200 Series HPLC system with binary pump. It comes with the followings:

G1379B Degasser
G1312A Binary pump
G1367B Hip-als
G1330B FC/Als therm
G1316A tcc
G1314B VWD
Agilent 1200 Hplc system with binary pump is currently not available for ordering, and it may not be in stock again.

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Used Agilent 1100 LC System with Binary Pump & DAD  

Manufacturer: Agilent Technologies
Condition: As Is
Includes:

1100 Solvent Tray
1100 Degasser
1100 Binary Pump
1100 Autosampler
1100 Diode Array Detector (DAD)
G2170 Data System
HPIB cables and CAN cables
Manuals
Used Agilent 1100 LC System with Binary Pump & DAD is currently not available for ordering, and it may not be in stock again.

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Used Agilent 1260 Infinity HPLC System with DAD,Quat Pump,ALS,TCC  

This Agilent 1290 Infinity Series HPLC System included:
1260: DAD
1260: Quat Pump (with built in Degasser); 600 Bar
1260: ALS
1260: TCC
W7 Pro Computer
Chemstation Software
 

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Used Agilent 1200 Hplc system with Degasser,Bin pump ,Hip-als ,MWD,TCC  

The binary pump comprises two identical pumps integrated into one housing. It provides gradient generation by high-pressure mixing The binary pump is based on a two-channel, dual-piston in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by two pump assemblies which can generate pressure up to 400 bar.

Each channel comprises a pump assembly including pump drive, pump head, active inlet valve which has a replaceable cartridge, and outlet valve. Both channels are connected in a low-volume mixing chamber which is connected by a capillary coil to a damping unit and a mixer. A purge valve including a PTFE frit is fitted at th

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USED AGILENT HP 1200 HPLC Series SYSTEM  

We offer an Agilent Technologies 1200 Series HPLC System in excellent working condition. Includes:
    •    Tested and Verified in Good Working Condition prior to ship
    •    G1322A Agilent 1200 Micro Vacuum Degasser
    •    Your choice Binary or Quaternary Pump
    •    Many AutoSamplers to choose from
    •    G1316B Agilent 1200 Thermostatted Column Compartment
    •    G1315D Agilent 1200/1260 DAD Diode Array Detector
    •    Agilent Solvent tray and bottles
    •    All fittings, cables, an

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Agilent 1260 Infinity II LC System  

Infinitely more confident.
The new 1260 Infinity LC sets higher standards in performance and value to give you more confidence in your results. The 600 bar power range combines with 80 Hz UV detector speeds and up to 10 times higher sensitivity. That‘s true UHPLC performance! At the same time you can run your existing HPLC methods completely unchanged. In short, the best solution for any HPLC or UHPLC application.
And within the price range of a typical HPLC!


Raising the HPLC standard 

The 1260 Infinity LC raises the bar in HPLC! With a 600 bar power range up to 5 mL/min for isocratic, quaternary and binary systems  and 200 bar up to 10 mL/min for isocratic and quaternary systems – plus 80 Hz detector speeds and 10 x higher sensitivity, you will reach new levels of versati

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Hplc Agilent Technologies 1260 Infinity II LC System  

Infinitely more confident.
The new 1260 Infinity LC sets higher standards in performance and value to give you more confidence in your results. The 600 bar power range combines with 80 Hz UV detector speeds and up to 10 times higher sensitivity. That‘s true UHPLC performance! At the same time you can run your existing HPLC methods completely unchanged. In short, the best solution for any HPLC or UHPLC application.
And within the price range of a typical HPLC!


Raising the HPLC standard 

The 1260 Infinity LC raises the bar in HPLC! With a 600 bar power range up to 5 mL/min for isocratic, quaternary and binary systems  and 200 bar up to 10 mL/min for isocratic and quaternary systems – plus 80 Hz detector speeds and 10 x higher sensitivity, you will reach new levels of ve

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Used Agilent 1290 Infinity Series HPLC System  

This Agilent 1290 Infinity Series HPLC System included:
1290 Infinity Binary Pump
1290 Infinity Autosampler
1290 Infinity FlexCube
1290 Infinity Thermostat
1290 Infinity Thermostatted Column Compartment
1290 Infinity Tray
1200 Instant Pilot
Computer loaded with Chemstation Software
 

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Hplc Agilent Technologies 1260 Infinity II LC System Brochures  

Prove Solutions for Clnical Research and Forensic Toxicology
See Agilent's clinical research products and services for specific research applications.

16-Sep-2016
1.71 MB
PDF


Agilent InfinityLab. Maximize your LC Workflow Efficiency.
Agilent InfinityLab. Maximize your LC Workflow Efficiency.

15-Aug-2016
572.54 KB
PDF


Agilent InfinityLab. Maximize your LC workflow Efficiency.
Agilent InfinityLab. Maximize your LC workflow Efficiency.

26-Jul-2016
884.18 KB
PDF


Agilent InfinityLab. Maximum efficiency for your liquid chromatography workflows.
Agilent InfinityLab. Maximum efficiency for your liquid chromatography workflows.

20-Jun-2016
4.26 MB
PDF


Agilent InfinityLab Supplies for the Agilent 1260 Infinity II LC with Multisampler
Agilent InfinityLab S

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features Of Agilent 1260 Infinity II Variable Wavelength Detector:
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resoluti

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Agilent 1260 Infinity II Multiple Wavelength LC Detector  

Reliable, simultaneous quantification of primary compounds, by products and impurities
The diode array design of the Agilent 1260 Infinity II Multiple Wavelength Detector offers very low detector noise (less than ± 7 μAU) for precise quantification of trace levels, regardless of the number of signals recorded. Over a wavelength range from 190 to 950 nm, simultaneous detection of up to eight compound-specific wavelengths for optimum selectivity is provided. High-speed UV detection with up to 120 Hz data rates keeps pace with the analysis speed of fast LC.
Features:
Increased sensitivity and selectivity - with simultaneous acquisition of up to eight compound-specific wavelengths.
Low detection limits - low noise front-end electronics and special flow cell design deliver lowest detection

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Preventing Leaks In HPLC Analyses  

Leaks are a common problem in HPLC analyses. To minimize leaks in your system, avoid interchanging hardware and fittings from different manufacturers. Incompatible fittings can be forced to fit initially, but the separation may show problems and repeated connections may eventually cause the fitting to leak. If interchanging is absolutely necessary, use appropriate adapters and check all connections for leaks before proceeding. Highly concentrated salts (>0.2 M) and caustic mobile phases can reduce pump seal efficiency. The lifetime of injector rotor seals also depends on mobile phase conditions, particularly operation at high pH. In some cases, prolonged use of ion pair reagents has a lubricating effect on pump pistons that may produce small leaks at the seal. Some seals do not perform

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clogged column frit HPLC problem  

Leaks are a common problem in HPLC analyses. To minimize leaks in your system, avoid interchanging hardware and fittings from different manufacturers. Incompatible fittings can be forced to fit initially, but the separation may show problems and repeated connections may eventually cause the fitting to leak. If interchanging is absolutely necessary, use appropriate adapters and check all connections for leaks before proceeding. Highly concentrated salts (>0.2 M) and caustic mobile phases can reduce pump seal efficiency. The lifetime of injector rotor seals also depends on mobile phase conditions, particularly operation at high pH. In some cases, prolonged use of ion pair reagents has a lubricating effect on pump pistons that may produce small leaks at the seal. Some seals do not perform

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USED Agilent 1200 Series Nano HPLC system with Degasser , Nano Pump , Micro WPS ,TCC , DAD  

This HP/Agilent 1200 Series Nano HPLC system has just been moved from a working lab. It comes with the followings:


G1379B Degasser with Tray


G2226A Nano Pump 


G1377A Micro WPS


G1316A TCC


G1315B DAD


PC with Suitable software and all essential cables required for full operation


Fore more information, about USED Agilent 1200 Series Nano HPLC system with Degasser , Nano Pump , Micro WPS ,TCC , DAD please call.

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Waters Breeze 2 HPLC  

The Breeze™ 2 HPLC System delivers technology and performance in an affordable, compact, and user-friendly system platform. Complete with software, pump, detector and injector, the system comes pre-configured for different levels of HPLC operational needs.
From teaching purposes to daily analytical work, the Breeze 2 HPLC System integrates simplicity, sensitivity, accuracy, and reliability. It is ideal for any organization seeking a quality HPLC platform with limited budget and chromatography experience including university laboratories, government laboratories, or start-up companies.
The Waters® Breeze 2 HPLC System delivers routine analyses and robust performance day after day, providing the  onfidence you need to get the job done. Used in laboratories worldwide, chromatograp

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features :
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resolution gainin fast LC -at up to 120 Hz data acquisition r

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 Used Agilent 1200 series with Micro Degasser ,Quatenary Pump , Autosampler , VWD  

Whatever your liquid chromatography analysis needs are you will find your individual solution within the Agilent LC portfolio. The Agilent 1200 Series is the industry-leading standard, offering everything you need for analytical HPLC methodologies. From routine analysis through new product research and development in chemical analysis and pharmaceutical industries, the Agilent 1200 Series helps you achieve reliable and robust results for all your applications.

 LC system options:


Agilent 1200 series G1379B Micro Degasser
Agilent 1200 series G1311A Quatenary Pump
Agilent 1200 series G1329A Autosampler
Agilent 1200 series G1330B ALS Therm
Agilent 1200 series G1316A  Infinity Thermostatted Column Compartment
Agilent 1200 series G1314 Variable Wavelength Detector (VWD)

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Agilent 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

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Hplc Agilent Technologies 1290 Infinity II LC System  

Welcome to the infinitely better liquid chromatography system. Innovative new technology delivers more resolution per time,more sensitivity and more flexibilityThe debate is over. The advantages of smaller particles—speed, resolution and sensitivity—were developed 40 years ago. The technology took a giant step forward in 2003 when Agilent introduced the first totally porous sub-2 μm particle columns, the ZORBAX Rapid Resolution High Throughput (RRHT) columns. The availability of other advanced particle columns provided additional ways to achieve further gains in separation power per time by using higher flow rates.
Today, about a dozen vendors offer dedicated UHPLC systems. All of them operate at different pressures, flow rates, system volumes, temperatures and detector sensit

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HPLC (high performance liquid chromatograph ) Instrumentation  

Main components in an HPLC system include the solvent reservoir, or multiple reservoirs, a high-pressure pump, a column, injector system and the detector.

The reservoir holds the solvent, which is referred to as the mobile phase because it moves. There are usually a minimum of two reservoirs in a system, with each holding up to 1000 cc of solvent and usually fitted with a gas diffuser through which helium can be bubbled. A pump is used to generate a specified flow of the mobile phase. Although manual injection of samples is still possible, most HPLCs are now fully automated and controlled by computer. The injector, or auto sampler, introduces the solvent into a phase stream that carries the sample into the high pressure (up to 400 bar) column, which contains specific packing material nee

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Used Agilent 1100 Series HPLC with Multiple Wavelength Detector System  

HPLC System with Computer
Agilent 1200 HPLC system includes,


G1315B DAD Detector


G1316A Colcom column compartment 


G1329A ALS Auto sampler 


G1311A Quat Pump 


 G1322A Degasser 


G1314D VWD Detector 


G4208A Controller 


Tray, Computer, Monitor, Column rack and Documentation


Agilent 1200 HPLC Complete System with Computer​  is currently not available.

 
s

ادامه مطلب  

 Used Agilent 1200 HPLC Complete System with Computer  

HPLC System with Computer
Agilent 1200 HPLC system includes,


G1315B DAD Detector


G1316A Colcom column compartment 


G1329A ALS Auto sampler 


G1311A Quat Pump 


 G1322A Degasser 


G1314D VWD Detector 


G4208A Controller 


Tray, Computer, Monitor, Column rack and Documentation


Agilent 1200 HPLC Complete System with Computer​  is currently not available.

 
s

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used Agilent 1200 Series HPLC LC System  

This listing is for an Agilent 1200 DAD HPLC System
The systems consists of the following modules

 1200 G1379B Degasser
1200 G1312A Bin Pump
11200 G1329A ALS
1200 G1316A TCC
1200 G1315B DAD
Windows desktop computer with Chemstation software loaded
Bottles, cables, and all necessary power cords

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USED Agilent 1200 Series HPLC system with Diode Array Detector & Degasser  

This used  HP / Agilent 1200 Series HPLC system comes with the followings:


Solvent Tray


Agilent 1200 Series G1379B Degasser


Agilent 1200 Series G1312A Bin Pump


Agilent 1200 G1367B HiP ALS Autosampler


Agilent 1200 G1330B FC/ALSTherm


Agilent 1200 Series G1316A TCC 


Agilent 1200 Series G1315B DAD 


PC with Suitable software and all essential cables required for full operation


Fore more information, about used Agilent 1200 Series HPLC system with Diode Array Detector & Degasser please call.

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 Used Agilent 1200 Series Nano LC  

1200 Series G1379B Degasser
1200 Series G2226A Nano pump
1200 Series G1330B Thermostatted controller

1200 Series G1377A Micro well plate Autosampler

1200 Series G1316B Column Compartment

1200 Series 4208A Gameboy controller

1200 Series Solvent Tray
used Agilent 1200 series Nano LC is currently not available for ordering, and it may not be in stock again.

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Features Of Agilent 1290 Infinity II LC System  

Maximize Analytical Efficiency
More chromatographic resolution – specially designed components in the sample flow path achieve lowest system dispersion.
Higher peak capacity for challenging separations ­– switch easily between single dimension UHPLC and the ultimate chromatographic power of 2D-LC.
Lower carryover for uncompromised data quality – multiwash capabilities of the 1290 Infinity II Multisampler reduce carryover to less than 10 ppm even for challenging compounds such as chlorohexidine.
Unique detection capabilities - combine lowest detection limits with an ultra-wide dynamic range by using the new 1290 Infinity II HDR- DAD or 1290 Infinity II ELSD.
Maximize Instrument Efficiency
Faster injection cycles with dual-needle injection and smart overlapped injections off

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principle of hplc:High Performance Liquid Chromatography (HPLC)  

High performance liquid chromatography (HPLC) has become a very versatile and powerful separation and analytical method over the years. It is an advanced form of liquid chromatography (LC).
Instead of introducing the solvent into the column and allowing it to drip down under the influence of gravity, in HPLC the sample is forced through the column under high pressures of nearly 400 atm, resulting in faster and more efficient separation.
This technique is also called high pressure liquid chromatography.

The Principle of HPLC
HPLC follows the same basic principle as chromatography. Different components in the sample have varying affinities to the adsorbent material. This causes a difference in the flow rate for each component which leads to their separation as they come out of the column.

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Principle of HPLC:High Performance Liquid Chromatography (HPLC) : Principle, Types, Instrumentation and Applications  

Chromatography is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition. This involves a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it. Sample components that display stronger interactions with the stationary phase will move more slowly through the column than components with weaker interactions. This difference in rates cause the separation of variuos components. Chromatographic separations can be carried out using a variety of stationary phases, including immobilized silica on glass plates (thin-layer chromatography), volatile gases (gas chromatography

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Mobile Phase (eluent) In Column Chromatography  

The mobile phase or eluent is either a pure solvent or a mixture of different solvents. It is chosen so that the retention factor value of the compound of interest is roughly around 0.2 - 0.3 in order to minimize the time and the amount of eluent to run the chromatography. The eluent has also been chosen so that the different compounds can be separated effectively. The eluent is optimized in small scale pretests, often using thin layer chromatography (TLC) with the same stationary phase.
There is an optimum flow rate for each particular separation. A faster flow rate of the eluent minimizes the time required to run a column and thereby minimizes diffusion, resulting in a better separation. However, the maximum flow rate is limited because a finite time is required for the analyte to equi

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principle of hplc:How Does High Performance Liquid Chromatography Work?/what is Detector,Chromatogram?  

How Does High Performance Liquid Chromatography Work?

The components of a basic high-performance liquid chromatography [HPLC] system are shown in the simple diagram in Figure E.
A reservoir holds the solvent [called the mobile phase, because it moves]. A high-pressure pump [solvent delivery system or solvent manager] is used to generate and meter a specified flow rate of mobile phase, typically milliliters per minute. An injector [sample manager or autosampler] is able to introduce [inject] the sample into the continuously flowing mobile phase stream that carries the sample into the HPLC column. The column contains the chromatographic packing material needed to effect the separation. This packing material is called the stationary phase because it is held in place by the column hardware.

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